Use of Ocudox™ or hypochlorous acid to inactivate SARS-CoV-2 as an alternative to alcohol-based products

Objective: The main route of entry SARS-CoV-2 which responsible COVID-19 is through the nasal, oral, or conjunctival mucosa and droplets aerosols transmit virus. Even if transmission fomites considered unlikely, hand hygiene important to minimize this other infections, especially in certain scenarios as healthcare facilities crowded places public transport social events. Material methods: In study, we assess compare virucidal efficacy HOCl solutions against at different time-points. a nasopharyngeal swab collected from an 89-year-old male patient during March 2020 Catalonia, Spain (GISEAD ID EPI_ISL_510689). virus was propagated Vero E6 cells (ATCC CRL-1586) cultured Dulbecco’s modified Eagle medium (DMEM). A total 24 plastic plates (35 mm SPL Ref. PLC20035) for first 12 second one, were prepared. One day before titration, 96-well prepared with cells. two replicates five 10-fold serial dilutions each sample performed. Plates incubated 37°C 5% CO2 7 days, afterward, SARS-CoV-2-induced cytopathic effect (CPE) determined under light microscope Results: by means TCID50/mL found after treatment concentrations times tested. Logarithmic reduction (logR) correspondent percentage inactivation (%) calculated compared untreated controls (desiccated water media). All tested able partially inhibit Conclusion: Our studies showed that products represent valid alternative inactivate potentially present skin surfaces.